What is the beta globin gene locus?
The human β-globin locus is composed of five genes located on a short region of chromosome 11, responsible for the creation of the beta parts (roughly half) of the oxygen transport protein Haemoglobin. This locus contains not only the beta globin gene but also delta, gamma-A, gamma-G, and epsilon globin.
What does the locus control region do?
Locus control regions (LCRs) are operationally defined by their ability to enhance the expression of linked genes to physiological levels in a tissue-specific and copy number–dependent manner at ectopic chromatin sites.
What is the gene locus for the HBB gene?
HBB protein is produced by the gene HBB which is located in the multigene locus of β-globin locus on chromosome 11, specifically on the short arm position 15.4.
What is locus control region in transcription?
Locus control regions (LCRs) are groups of transcription regulatory elements that regulate clusters of genes often involved in cellular differentiation and development (Dean, 2006; West and Fraser, 2005).
How do the genes in the β globin locus differ from each other quizlet?
How are the genes in the β-globin locus different from each other? They are expressed at different times in development. Which disease results from deletion of the β-globin gene cluster LCR? Which of the following is an example of a recombinant DNA molecule?
Where is beta globin gene located?
chromosome 11
The β-globin gene cluster occupies a region of approximately 17 kb on the short arm of chromosome 11. Each of its constituent genes, their flanking regions, and large stretches of the regions between them have been sequenced.
Where is locus control region?
The locus is located on chromosome 11 (11p15. 5) and contains five developmentally regulated erythroid cell-specific genes arranged in the order in which they are expressed during development (5′-ɛ-Gγ-Aγ-δ-β-3′) and an upstream regulatory region characterized by five DNase I-hypersensitive sites (HSs; see Fig. 1).
What does LCR mean in genetics?
A locus control region (LCR) is a long-range cis-regulatory element that enhances expression of linked genes at distal chromatin sites. It functions in a copy number-dependent manner and is tissue-specific, as seen in the selective expression of β-globin genes in erythroid cells.
How do the genes in the β-globin locus differ from each other quizlet?
Is beta globin a housekeeping gene?
Presently, housekeeping genes (Beta globin, GAPDH) are used in molecular diagnosis to verify that the PCR conditions are optimum, and are thus known as amplification controls [1], [2], [3], [4].
What is an LCR and what is its role in a chromosomal domain?
How can microarrays differentiate between a wild type allele and a disease allele that differ at only one nucleotide quizlet?
How can microarrays differentiate between a wild-type allele and a disease allele that differ at only one nucleotide? Oligonucleotides hybridize with the two alleles differently. Which process(es) can generate recombinant gametes?
Which of the following accurately describes the relationship between orthologous Paralogous and homologous genes?
Which of the following accurately describes the relationship between orthologous, paralogous and homologous genes? Multiple choice question. Both homologous and orthologous genes are also paralogous.
What is the difference between alpha and beta-globin?
Beta-globin is a component (subunit) of a larger protein called hemoglobin, which is located inside red blood cells. In adults, hemoglobin normally consists of four protein subunits: two subunits of beta-globin and two subunits of a protein called alpha-globin, which is produced from another gene called HBA.
What is the order of these Hb globin genes in the Hb gene cluster?
The human β-globin gene cluster consists of five genes arranged in chromosome 11 in the same order in which they are expressed during development: 5′-ɛ-, Gγ -, Aγ-, δ-, and β-globin gene (8) (Fig. 1).
What is the function of beta globin?
The beta globin protein is one of the subunits of hemoglobin, a protein necessary for the oxygen-carrying function of red blood cells. People with the sickle cell mutation in both copies of the HBB gene produce proteins that clump together and lead to changes in the shape and behavior of red blood cells.
What information Cannot be determined from the DNA sequence of a cDNA clone?
med genetics ch9
| Question | Answer |
|---|---|
| What information CANNOT be determined from the DNA sequence of a cDNA clone? A)exon sequences B)sequence of the promoter C)similarity to previously identified sequences D)amino acid sequence of the encoded polypeptides | B) sequence of the promoter |
How could PCR be used for the detection of a SNP quizlet?
How could PCR be used for the detection of a SNP? The PCR reaction products would need to be sequenced. at least some sequence information about the region to be amplified. If a single DNA molecule is amplified by PCR, how many DNA molecules will there be after six cycles?
What is the difference between paralogous and orthologous genes?
Orthologous (or homologous) genes are found in different organisms, but are derived from a single common ancestral gene present in the common ancestor of those organisms. Paralogous genes are genes present in a particular organism that are related to each other through a gene duplication event.
What is the difference between orthologous genes and homologous genes?
“By definition, orthologs are genes that are related by vertical descent from a common ancestor and encode proteins with the same function in different species. By contrast, paralogs are homologous genes that have evolved by duplication and code for protein with similar, but not identical functions.”
What is the beta globin gene cluster?
The β-globin gene cluster located at 11p15. 5 comprises one pseudogene and five genes whose expression undergoes two critical switches: the embryonic-to-fetal and fetal-to-adult transition. HBD encodes the δ-globin chain of the minor adult hemoglobin (HbA2), which is assumed to be physiologically irrelevant.
Where is beta-globin gene located?
What is beta-globin gene sequencing?
Beta-globin gene (HBB) sequencing can be used to identify hemoglobin variants and the most common beta thalassemia sequence variants, including beta plus and beta zero thalassemias.
Why is cDNA better than genomic DNA?
Advantages of cDNA over Genomic DNA
No introns: Eukaryote genes commonly contain introns (non-coding sequences). These are removed after mRNA synthesis so cDNA contains no introns. This means that a cDNA copy of a gene can be isolated as a single, intron-free fragment.
Why is cDNA used instead of mRNA?
cDNA is a more convenient way to work with the coding sequence than mRNA because RNA is very easily degraded by omnipresent RNases. This the main reason cDNA is sequenced rather than mRNA. Likewise, investigators conducting DNA microarrays often convert the mRNA into cDNA in order to produce their probes.