How do you differentiate Caco-2 cells?
Standard methods used to differentiate Caco-2 cells involve seeding them on culture inserts fitted with polycarbonate filters, and allowing spontaneous differentiation to proceed for two to three weeks in culture medium containing 10% or 20% foetal bovine serum (FBS) in both the apical (AP) and basolateral (BL) …
What do Caco-2 cells do?
Caco-2 cells are a cell model of small intestinal enterocytes (Sambuy et al., 2005). Caco-2 cells allow independent examination of the effects of calcium on iron absorption of the food matrix and calculation of molar ratios of calcium:iron.
What is the Caco-2 cell line?
Caco-2 [Caco2] are epithelial cells isolated from colon tissue derived from a 72-year-old, White, male with colorectal adenocarcinoma. This cell line is a suitable transfection host and has applications in cancer and toxicology research.
How long does it take for Caco-2 cells to adhere?
CACO-2 cells will reach full confluence after approximately 4 days in culture when seeded at 2-4×10,000 cells/cm². CACO-2 cells will require 17-21 days in culture in order to differentiate and demonstrate the desired characteristics of small intestine enterocytes [9].
What is Caco-2 permeability assay?
Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs. The Caco-2 cell line is derived from a human colon carcinoma.
What are calu 3 cells?
Description. Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene.
What is Caco-2 called?
IUPAC name. Calcium carbonate. Other names. Aragonite; calcite; chalk; lime; limestone; marble; oyster; pearl.
What is the full form of Caco-2?
calcium carbonate. (redirected from CaCO2)
Do Caco-2 cells produce mucus?
However, Caco-2 cells are mostly reported as non/low-mucus producing cells and do not or hardly express mucin-related genes, such as MUC-2 and MUC-625.
How do you thaw Caco-2 cells?
Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes.
What is Caco permeability?
Caco-2 permeability assay to investigate intestinal permeability. Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs.
How do you measure drug permeability?
There is a variety of methods that have been developed to assess drug permeation across the gastrointestinal tract. The methods include in situ perfusion through isolated intestinal segments, ex vivo diffusion across tissues, and in vitro permeation through cell monolayers or artificial membranes.
What does calu 3 stand for?
Cultured Human Airway Epithelial Cells
Cultured Human Airway Epithelial Cells (Calu-3): A Model of Human Respiratory Function, Structure, and Inflammatory Responses.
What are HuH 7 cells?
HuH-7 (hereafter Huh7) is a permanent cell line established from male hepatoma tissue, which was surgically removed from a 57-year-old Japanese male in 1982 (Nakabayashi et al., 1982). Huh7 and its derivatives have been used as a convenient experimental substitute for primary hepatocytes.
What does caco2 stand for?
Caco-2 (from Cancer coli, “colon cancer”) is an immortalized cell line of human colorectal adenocarcinoma cells. It is primarily used as a model of the intestinal epithelial barrier. In culture, Caco-2 cells spontaneously differentiate into a heterogeneous mixture of intestinal epithelial cells.
What is the other name of CaCO3?
Calcium Carbonate
Calcium carbonate is a chemical compound with the formula CaCO3 formed by three main elements: carbon, oxygen, and calcium.
What is the chemical name of CaCO3?
Calcium carbonateCalcium carbonate / IUPAC ID
What are the mucus producing cells?
Mucus is secreted by the goblet cells and typically contains several major components. One of these, the mucins, gives the mucus its gel-like properties.
What is HT29 MTX?
Cell Line Description: HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and production of a mucous layer.
Why must thawing of cells be done quickly?
Cell freezing needs to occur at a slow, controlled cooling rate. In contrast, cell thawing works best when it is done quickly because the disappearance of ice around the cell does not have the same damaging effects as ice formation during cryopreservation.
How do you thaw in cell culture?
Thawing Cells: Cell Culture Basics – YouTube
What factors affect permeability of drug?
Drugs may cross cell membranes by passive diffusion, facilitated passive diffusion, active transport, and pinocytosis. The drug’s physicochemical properties (such as size and lipophilicity), as well as membrane-based efflux mechanisms, can lead to poor permeability.
What are calu cells?
What are BEAS 2B cells?
The BEAS-2B cell line was originally established as a human non-tumorigenic lung epithelial cell line derived from a human lung tissue and has been extensively used as an in vitro non-tumorigenic lung epithelial model in a large variety of studies in association with lung carcinogenesis for over 30 years.
What is the difference between HepG2 and Huh-7?
Despite the generally darker apoB100 bands in the density fractions from the conditioned medium of HepG2 cells, when normalized to cell protein, Huh-7 cells actually secrete more apoB100 mass (0.59 ± 0.11 ng/µg cell protein/h) than HepG2 cells (0.27 ± 0.03 ng/µg cell protein/h).