Does NP-40 denature proteins?
A weaker detergent such as NP-40 can be used to solubilize proteins but not denature them.
How do I make a NP-40 lysis buffer?
1. Prepare 1% NP40 lysis buffer (LB). (To make 50 ml of 1% NP40 lysis buffer, add 5 ml of 10% NP40, 500 μl of 1 mM EDTA, 1.5 ml of 150 mM NaCl, and 1 ml of 20 mM Tris–Cl, pH 7.4; and bring the total volume to 50 ml using dH2O.) 2.
What is NP-40 lysis buffer?
Description. NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting. It can also be used as a wash buffer for immunoprecipitation reactions.
Is NP-40 same as igepal?
Nonidet P-40 and IGEPAL CA-630 are the same, those are merely different brand names. According to the manufacturer, Sigma-Aldrich, the two compounds are “chemically indistinguishable”.
Does NP-40 break nuclear membrane?
These buffers can be used for co-IPs. NP-40 and Triton X-100 will not lyse nuclear membranes. After lysis, pellet the nuclei by centrifugation and transfer the supernatant to a new tube. If you wish to isolate both the nuclear and soluble fractions, resuspend the nuclear pellet in RIPA buffer.
What is the function of NP-40?
NP-40 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability.
How do you dilute NP-40?
Directions: 1) Mix 20 ml of NP-40 with 80 ml of ddH2O by stirring. 2) Store at 4°C. Note: Nonidet P-40 is chemically identical to Igepal-CA-630.
What is NP-40 used for?
NP-40 is often used to break open all membranes within a cell, including the nuclear membrane. To break only the cytoplasmic membrane, other detergents such as IGEPAL CA-630 can be used. NP-40 has applications in paper and textile processing, in paints and coatings, and in agrochemical manufacturing.
What does NP-40 stand for?
NP-40 is an ethoxylated nonylphenol for non-ionic surfactants and can act as emulsifier and demulsifier agent. NP-40. Names. Other names. Polyethylene glycol nonylphenyl ether; Nonyl phenoxypolyethoxylethanol; Nonoxynol-40.
Does NP-40 lyse nuclear membrane?
NP-40 and Triton X-100 will not lyse nuclear membranes. After lysis, pellet the nuclei by centrifugation and transfer the supernatant to a new tube. If you wish to isolate both the nuclear and soluble fractions, resuspend the nuclear pellet in RIPA buffer. NP-40 is also marketed under the name Igepal CA-630.