What type of cancer is MDA-MB-231?
The MDA-MB-231 cell line is an epithelial, human breast cancer cell line that was established from a pleural effusion of a 51-year-old caucasian female with a metastatic mammary adenocarcinoma1 and is one of the most commonly used breast cancer cell lines in medical research laboratories.
What is the difference between MCF-7 and MDA-MB-231?
The MCF-7 cell line has functional estrogen and EGF receptors, is dependent on estrogen and EGF for growth, and is uninvasive, while MDA-MB-231 cells are a model for more aggressive, hormone-independent breast cancer.
What is the difference between MDA-MB-231 and MDA-MB-468?
MDA-MB-157, MDA-MB-231, and BT-549 cells are characterized as triple-negative/basal-B mammary carcinoma, while the MDA-MB-468 cells are characterized as triple-negative/basal-A mammary carcinoma.
What does MDA MB stand for?
MDA-MB 231 (>> Co-occurring Abbreviation) Long Form: M.D. Anderson – Metastatic Breast 231. Abbreviation Variation.
Do MDA-MB-231 cells express HER2?
MDA-MB-231 cells express low levels of HER2. B, Levels of soluble HER2 shedding by multiple cancer cell lines as determined by ELISA.
What is a triple negative cancer?
What Is Triple-Negative Breast Cancer? Triple-negative breast cancer is a kind of breast cancer that does not have any of the receptors that are commonly found in breast cancer. Think of cancer cells as a house. The front door may have three kinds of locks, called receptors— One is for the female hormone estrogen.
Is MCF7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
Does MCF-7 express HER2?
Discoveries in breast cancer pathology through experiments using MCF-7 cells. MCF-7 sub-lines demonstrate a wide divergence in the relative expression of ER, PR and HER2.
Do MDA MB 231 cells express HER2?
What is MDA MB 468 cell line?
MDA-MB-468 is a cell line with epithelial morphology that was isolated from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast. These cells have applications in breast cancer and immuno-oncology research.
What is the doubling time for MDA-MB-231?
about 38h
Hi Nairrita, the doubling time of MDA-MB-231 cells is about 38h according to ATCC. I usually start with the density of 7,500 cells/cm2 in 96-well plate for 3 days MTS assay.
How do you freeze MDA-MB-231 cells?
Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO. 1. Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1–2 minutes by gentle agitation in a 37°C water bath.
What does HER2 mean?
HER2-positive breast cancer is a breast cancer that tests positive for a protein called human epidermal growth factor receptor 2 (HER2). This protein promotes the growth of cancer cells.
What is the deadliest form of breast cancer?
Triple-negative breast cancer (TNBC) is considered an aggressive cancer because it grows quickly, is more likely to have spread at the time it’s found, and is more likely to come back after treatment than other types of breast cancer.
How long can you live with triple-negative?
It’s one of the most challenging breast cancers to treat. But researchers are making steady progress toward more effective treatments. Overall, 77% of women who have triple negative breast cancer are alive five years after diagnosis.
How fast do MCF-7 cells grow?
MCF-7 human mammary carcinoma cells were inoculated into 150-sq cm flasks at 3 X 10(5) cells/flask, and after a lag period of about 48 hr, these cells grew exponentially for 5 days with a mean population doubling time of about 24 hr.
Is MCF-7 invasive?
Though MCF7 is categorized as poorly invasive, its migration in the 2D surface is found to be comparable to the highly invasive MDA MB 231 in the current study.
Are MCF-7 cells metastatic?
MCF-7 is a poorly-aggressive and non-invasive cell line (9), normally being considered to have low metastatic potential (8).
What is the doubling time for MDA MB 231?
What is A549 cell line?
A549 cells are lung carcinoma epithelial cells that constitute a cell line. A549 is one of the commonly used cell lines for a wide range of research applications. In this article, we will provide you with all the information you will need to work with these cells.
How do you freeze MDA MB 231 cells?
What is hepg2 cell line?
Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.
How long can you live with HER2?
The survival rate among patients differ based on the molecular subtype and stage. The survival rate at four years among women with HR+/HER2− is estimated to be 92.5%, followed by HR+/HER2+ at 90.3%, HR−/HER2+ at 82.7%, and HR−/HER2− at 77.0% [1].
What is worse HER2-positive or negative?
Breast cancer cells with higher than normal levels of HER2 are called HER2-positive. These cancers tend to grow and spread faster than breast cancers that are HER2-negative, but are much more likely to respond to treatment with drugs that target the HER2 protein.
What type breast cancer has the highest recurrence rate?
Research suggests that estrogen receptor-positive breast cancer is more likely to come back more than five years after diagnosis.