How do you use a Rainin pipette?
Make sure you apply consistent pressure on the plunger at the first stop point or hold. Position also pay attention to a consistent speed and smoothness when pressing and releasing.
How do you set up a Rainin pipette?
Basic multi dispense push right to go into setting aliquot volume is lit press the joystick to select on this volume pipette push right to increase or left to decrease by 10 microliters push.
How do you calibrate a pipette Rainin?
First after preparing the vessel and tearing the balance adjust the pipette to 100% of its nominal volume. And load the manufacturers recommended tip next pre-rinse the tip by aspirating.
Are Rainin pipettes autoclavable?
The shaft and tip ejector are autoclavable: 121°C, 1 atm, 15–20 minutes.
How often should Rainin pipettes be calibrated?
A good rule of thumb is to choose a valid calibration company that complies with testing standards and includes preventative maintenance. After that, you may wish to have your pipette performance tested every 6 months, especially if you use your pipettes daily.
How do you clean a Rainin pipette?
Rinse thoroughly with distilled water and allow to dry. Open the pipette and place the contaminated parts in a strong detergent or cleaning solution. Rinse several times with distilled water and allow to dry.
How do you use Rainin e4 XLS?
Rainin E4 XLS, XLS+ pipette: Manual mode – YouTube
Do new pipettes need calibration?
Essentially, calibrating your new pipette purchase reduces the risk of any potential pipetting errors prior to beginning to use your new pipette. If there is any uncertainty of the calibration of your new pipette, you could be introducing unknown errors into your measurements.
How do you reuse pipette tips?
Load the tips into an existing pipette tip rack by placing a refill insert into an existing pipette tip rack and firmly press down to dispense. These refill inserts come stacked in a cardboard sleeve that unfolds and reseals for efficient storage.
How do I know if my pipette is accurate?
The most common way to check your pipette accuracy is by weighing water. The density of water is 1 g/mL. This means that every microliter (µL) should weigh exactly 0.001 g using a high-precision balance.
How much does it cost to calibrate a pipette?
They are also making large profits on each pipette – an average price for onsite calibration is $25 per pipette, based on our estimations. However, the time spent on each pipette can be as little as 6 minutes.
How often should pipettes be cleaned?
Cleaning the outer surface of your pipette is quick and easy, and should be part of your daily routine. Wiping them with a lint-free cloth lightly soaked with 70 % ethanol should be sufficient, but do check the chemical compatibility of the pipettes beforehand.
How do you use an electronic pipette?
How to pipette with an electronic pipette – YouTube
What happens if a pipette is not calibrated?
Pipettes that are not calibrated at regular intervals produce questionable measurements, and the integrity of any results and data will be compromised. Resources and funding are often scarce for laboratories, and untrustworthy results can have a significant impact on the success of the company.
Can you wash and reuse pipette tips?
Yes. Conical tubes and even pipette tips can be washed and/or autoclaved and reused. Pipette tip boxes can be repurposed to hold other lab supplies or as containers for Western blots.
Can you use the same pipette tip twice?
When in doubt, throw it out. If you are ever questioning whether a new tip is necessary, use the new tip. Using a few extra pipette tips is always better than ruining an entire experiment with contamination.
Do I need to calibrate a new pipette?
The Clinical and Laboratory Standards Institute (CSLI) recommends that pipettes (single and multi-channel) and automated liquid handlers be calibrated every 3 to 6 months.
How can I improve my pipetting accuracy?
How good are you?
- Pre-wet the pipette tip.
- Immerse the tip to the proper depth during aspiration.
- Pause consistently after aspiration.
- Use consistent plunger pressure and speed.
- Pull the pipette straight out.
- Examine the tip BEFORE dispensing a sample.
- Examine the tip AFTER dispensing a sample.
- Use standard mode pipetting.
How do you calibrate a pipette yourself?
Pipette Calibration – YouTube
How often should I calibrate my pipettes?
every 3 to 6 months
The Clinical and Laboratory Standards Institute (CSLI) recommends that pipettes (single and multi-channel) and automated liquid handlers be calibrated every 3 to 6 months. A minimum of two volumes must be tested (nominal and lowest setting) with ten replicates at each volume.
What do you rinse a pipette with?
When you are finished with the pipet in your experiment, rinse with distilled water.
Why is an electronic pipette better?
Precision & Comfort: Using an electronic pipette significantly reduces the opportunity for human errors such as contamination of the workplace and irregular amounts of fluid in each pipette.
Why thumb is not used in pipetting?
You can not accurately control the flow of liquid from a pipette with your thumb. Never use your thumb to control release of liquid from a pipette. When using pipette with both upper and lower calibration marks, the volume delivered is controlled by the user.
How often do pipettes need to be calibrated?
every 3-6 months
According to the Clinical & Laboratory Standards Institute (CLSI), you should calibrate your pipettes every 3-6 months or before they’re put into service. If you fail to do so, then pipette readings may become inaccurate and unreliable.
How do you clean a transfer pipette?
Open the pipette, immerse the parts in a detergent solution, such as Deconex® 12 Basic. Rinse well with distilled water and allow to dry. DNA can be eliminated by immersing pipette parts in at least 3% (w/v) sodium hypochlorite for at least 15 minutes (2,3). Rinse well with distilled water and allow to dry.