What is needed for reverse transcription?
To initiate reverse transcription, reverse transcriptases require a short DNA oligonucleotide called a primer to bind to its complementary sequences on the RNA template and serve as a starting point for synthesis of a new strand.
What is required for reverse transcription?
How is reverse transcriptase made?
The process of reverse transcription generates, in the cytoplasm, a linear DNA duplex via an intricate series of steps. This DNA is colinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA (Fig.
What is the primer for reverse transcriptase?
DNA oligonucleotide
To initiate reverse transcription, reverse transcriptases require a short DNA oligonucleotide called a primer to bind the RNA template and serve as a starting point for synthesis of a new strand.
Does EDTA inactivate rnase?
Unlike many DNases, RNases do not require divalent cations for activity and thus cannot be easily inactivated by the inclusion of ethylenediaminetetraacetic acid (EDTA) or other metal ion chelators in buffer solutions.
Does EDTA inhibit RNase?
What is the source of the RNase H buffer?
The 10X RNase H Buffer (cat. no. B9220) contains 500 mM Tris-HCl, 750 mM KCl, 30 mM MgCl 2 and 100mM DTT; pH 8.3 at 25⁰C. OEM by QIAGEN offers bulk manufacturing of RNase H in custom formulations. The source of the protein is a recombinant E. coli strain carrying the RNase H (rnh) gene from E. coli.
What is RNA reverse transcriptase?
Reverse transcriptase is an RNA-dependent DNA polymerase that was discovered in many retroviruses such as human immunodeficiency virus (HIV) and avian myeloblastosis virus (AMV) in 1970.
What is the buffer for Superscript II reverse transcriptase?
SuperScript™ II Reverse Transcriptase (2,000 units total, at 200U/µL) is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT. Convenient, on-site access to the products you need. Learn more. Are SuperScript II and III RTs RNase H minus?
What is the pH of 10x RNase H buffer?
The 10X RNase H Buffer (cat. no. B9220) contains 500 mM Tris-HCl, 750 mM KCl, 30 mM MgCl 2 and 100mM DTT; pH 8.3 at 25⁰C. OEM by QIAGEN offers bulk manufacturing of RNase H in custom formulations.