What laser excites mCherry?

What laser excites mCherry?

561-nm laser

In addition, the 561-nm laser efficiently excites fruit fluorescent proteins such as mCherry.

What is mCherry used for?

mCherry is most commonly used as a fluorescent reporter, acting as a labeled tag for genes, cells, or organelles of interest. The emissive and fluorescent properties allow this protein to function as an intracellular probe, used especially for viewing constitutive gene expression.

Is RFP and mCherry the same?

mCherry is probably the most commonly used RFP variant. It is a monomeric red fluorescent protein with broad applicability as a fusion protein in various cell types.

Can I use mCherry and GFP?

Due to the spectral properties of GFP and mCherry, they are considered an ideal combination for co-localisation and co-expression experiments.

What is the difference between mCherry and GFP?

GFP is more sensitive to pH change than mCherry. The pH-dependent emission profile of GFP and mCherry was investigated by resuspending GFP and mCherry in phosphate buffers with a pH ranging from 4.4 to 9.1. The emission was then plotted against the pH value to obtain the profile.

What wavelength is mCherry excited at?

587 nm
mCherry has a single photon excitation peak at 587 nm and an emission peak at 610 nm [7].

Is mCherry toxic to cells?

Thus, the hexameric mCherry protein is not a potent toxin for just any cell type.

How do you detect mCherry?

It’ll depend on the flow cytometer you have access to but to best detect the mCherry signal excite with the yellow-green laser at 561 nm and detect in the PE-TexasRed channel with a 610/20 bandpass filter.

What is the half life of mCherry?

about 40 minutes
mCherry has a maturation half-time of about 40 minutes, whereas sfGFP becomes fluorescent much faster, on the order of a few minutes.

What is the best fluorescent protein?

Commonly used filter sets & relevant fluorescent proteins
In our hands the best fluorescent proteins for imaging with this set are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent protein such as iFP1. 4 or iFP2.

What color is mCherry?

red
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed.

Is tdTomato the same as mCherry?

The tandem dimer tdTomato is equally photostable but twice the molecular weight of mCherry, and may be used when fusion tag size does not interfere with protein function. mStrawberry is the brightest red monomer, but it is less photostable than mCherry, and should be avoided when photostability is critical.

What is the best red fluorescent protein?

mCherry is the most widely used and cited red fluorescent protein owing to its fast maturity, stability, and resistance to photobleaching. mCherry and DsRed-Monomer are ideal for tagging proteins with diverse functions and/or subcellular localization patterns.

What channel is mCherry?

Imaging