How do you differentiate C2C12?
Differentiation of C2C12 cells is achieved by replacing GM to differentiation media, DM [DMEM—high glucose no sodium pyruvate (Gibco), 2% horse serum (Gibco), 1% glutamine (Gibco), 1% pen/strep (Gibco)]. After 24 h in DM, fused cells should be visible. DM should be changed every 48 h.
What are C2C12 myoblasts?
C2C12 is a myoblast cell line that is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.
What type of cell is C2C12?
mouse myoblast cell line
C2C12 is an immortalized mouse myoblast cell line. The C2C12 cell line is a subclone of myoblasts that were originally obtained by Yaffe and Saxel at the Weizmann Institute of Science in Israel in 1977.
What do myotubes form?
Muscle fibers generally form through the fusion of precursor myoblasts into multinucleated fibers called myotubes. In the early development of an embryo, myoblasts can either proliferate, or differentiate into a myotube.
Are C2C12 cells adherent?
From the C2s the immortal subline C2C12 was selected (Blau et al., 1985). These cells differentiate well into myocytes under appropriate culture conditions given below. The cells are adherent in culture and are grown on Nunc delta surface plastic culture dishes.
How do myoblasts differentiate?
Myoblast differentiation is an ordered multistep process that includes withdrawal from the cell cycle and the expression of key myogenic factors leading to myoblast differentiation and fusion into multinucleated myotubes.
What are C2C12 cells used for?
Due to the presence of biochemical features, characteristic muscle proteins and cross striations, C2C12 cell model is often used in pharmaceutical science and biomedical research to examine skeletal muscle metabolism and differentiation.
Is C2C12 a stem cell?
(2015) developed the C2C12 cell line from the BubR1 hypomorphic mouse model of accelerated-aging (Baker et al., 2008); these cells are characterized by a subpopulation of muscle stem cells expressing p16Ink4a (Nozaki et al., 2015).
What are C2C12 myotubes?
C2C12 myotubes express contractile proteins and, when left to differentiate for an extended period of time, can spontaneously contract. These properties make C2C12 cells an invaluable tool for understanding the molecular biology of muscle development. However, these cells do not perfectly mimic in vivo muscle fibers.
What are myotubes?
Medical Definition of myotube
: a developmental stage of a muscle fiber composed of a syncytium formed by fusion of myoblasts.
How big are C2C12 cells?
At the time of the biophysical studies, mean myotube diameter was 12 microns (range 5-25 microns), and mean length was 290 microns (range 130-520 microns).
What can myoblasts differentiate into?
myotubes
In this setting, proliferating myoblasts can be induced to differentiate into myotubes by growing them to confluence and switching them to reduced serum (differentiation medium, DM). Under these conditions, most cells fuse to form myotubes after 4 d.
What is myoblasts function?
Myoblasts are the embryonic precursors of myocytes (also called muscle cells). Myoblasts differentiate into muscle cells through a process called myogenesis. During myogenesis, the myoblasts fuse into multi-nucleated myotubes, which later become the muscle fibers.
Is a myoblast a differentiated?
What is the function of a myoblast?
Myoblasts, the mononucleate precursor cells of skeletal muscle, can differentiate to form multinucleated muscle fibers capable of muscle contraction.
What does the myoblast do?
What does the term myoblast mean?
: an undifferentiated cell capable of giving rise to muscle cells.
How do myoblast cells control muscle cell development?
Each myofiber is the product of fusion of hundreds or thousands of mononucleated muscle cells known as myoblasts. Myoblast fusion is critical not only for skeletal muscle development during embryogenesis, but also for satellite cell-mediated muscle regeneration in adults [1,2].
What is a myoblast made of?
What is the role of a myoblast?
What is the myoblast function?