What does glutathione S-transferase do?
Glutathione S-transferases (GSTs) are ubiquitous enzymes that are encoded by a large gene family, and they contribute to the detoxification of endogenous or xenobiotic compounds and oxidative stress metabolism in plants.
Where is glutathione S-transferase found?
cytosol
The glutathione transferases (GSTs; also known as glutathione S-transferases) are major phase II detoxification enzymes found mainly in the cytosol. In addition to their role in catalysing the conjugation of electrophilic substrates to glutathione (GSH), these enzymes also carry out a range of other functions.
What is GST assay?
The Glutathione S-Transferase (GST) Assay Kit is intended for the measurement of total GST activity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma and erythrocyte lysates.
What does CDNB assay do?
The GST-mediated reaction of CDNB with glutathione produces a conjugate that is measured by absorbance at 340 nm using either a plate reader or a UV/Vis spectrophotometer. Assay results are available in less than 10 min for crude bacterial lysates, column eluates, or purified GST-tagged protein.
Is glutathione S transferase an antioxidant?
Antioxidant role of glutathione S-transferases: protection against oxidant toxicity and regulation of stress-mediated apoptosis.
Is glutathione S transferase a protein?
The glutathione S-transferases (GSTs) are an abundant family of dimeric proteins that have the capacity to conjugate glutathione (GSH) with a variety of compounds containing electrophilic centers.
What is GST activity?
The GST activity is determined by measuring the rate of produced conjugation between reduced glutathione and CDNB, which is proportional to the increase in absorbance at 340nm over time (ΔOD340nm/min). Kit Components. Cat.
Where is GST found?
In mammals, GST is found in all tissues and organs. In most tissues, GST appears in different forms. GST isoenzymes are primarily localized in the cytosol, but membrane-bound enzymes are also isolated.
Is GST a gene?
The glutathione S-transferase (GST) gene family encodes genes that are critical for certain life processes, as well as for detoxication and toxification mechanisms, via conjugation of reduced glutathione (GSH) with numerous substrates such as pharmaceuticals and environmental pollutants.
How do you calculate GST activity?
The GST activity is determined by measuring the rate of produced conjugation between reduced glutathione and CDNB, which is proportional to the increase in absorbance at 340nm over time (ΔOD340nm/min).