What is a Boyden chamber assay?
The Boyden chamber is a useful tool to study cell migration and cell invasion. It consists of a cylindrical cell culture insert nested inside the well of a cell culture plate. The insert contains a polycarbonate membrane at the bottom with a defined pore size.
What is invasion assay?
The Cell Invasion Assays offer a flexible, standardized, high-throughput format for quantitating the degree to which invasive cells penetrate a barrier consisting of basement membrane components in response to chemoattractants and/or inhibiting compounds.
What does invasion assay measure?
The in vitro invasion assay measures cell migration of endotheial cells through an extracellular matrix. Invasive migration is a major process in angiogenesis but also plays a significant role in pathological events such as cancer development and metastasis.
How is wound healing assay calculated?
The first method measures the change in the wound width (nanometres) over time. This width is the average distance between the two margins of the scratch. The second method calculates the change in wound area over time as a percentage of wound closure. These two methods can be time-consuming when performed manually.
What is Boyden chamber assay?
Boyden Chamber Assays. In these assays the Boyden chamber insert is coated on the underside with Collagen I or Fibronectin protein. Transmigration describes the migration of cells (usually leukocytes or tumor cells) through the vascular endothelium toward a chemoattractant.
What is the coating on the Boyden chamber insert?
In these assays the Boyden chamber insert is coated on the underside with Collagen I or Fibronectin protein. Transmigration describes the migration of cells (usually leukocytes or tumor cells) through the vascular endothelium toward a chemoattractant.
What types of cell invasion can cytoselect™ Boyden chamber assays measure?
Our CytoSelect™ Boyden Chamber Assays may be used to measure cell invasion as well as various types of cell migration: Chemotaxis describes cell migration based on chemicals in a cell’s surrounding environment. Haptotaxis is defined as cell migration along a gradient of extracellular matrix-bound chemoattractants.