What is Gal4 gene?
The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes. This protein represents a large fungal family of transcription factors, Gal4 family, which includes over 50 members in the yeast Saccharomyces cerevisiae e.g. Oaf1, Pip2, Pdr1, Pdr3, Leu3.
Does Gal4 bind to DNA?
Abstract. The DNA binding domain of the GAL4 transcription factor from yeast is located in the N-terminal 60 residues of the polypeptide of 881 amino acids.
What is the role of Gal4?
Gal4 activates transcription by recruiting coactivators and the general transcription machinery to promoter regions through its activation domain (Fig 1). Important questions remain as to what are the functional targets of Gal4 and whether it contacts one or many proteins during the process of recruitment.
What is Gal4 used for?
The Gal4/UAS system
It is a Drosophila geneticist’s main workhorse to turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression.
How does Gal4 work?
Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then recruits transcription machinery to the site to induce gene expression. Thus, genes and siRNA encoded downstream of the UAS sequence are only expressed when Gal4 is expressed.
Is Gal4 an activator?
Yeast Gal4, one of the first known acidic activators, is responsible for the regulation of genes involved in galactose catabolism (41).
Is Gal4 a protein?
The UAS-GAL4 system is a method of activating gene expression in Drosophila (Figure 26.2). The GAL4 protein, derived from yeast, serves as the transcriptional activator in this system.
Is GAL4 an activator?
What is a GAL4 driver line?
The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs to manipulate neuronal function.
Is GAL4 a protein?
What types of genes does the Gal4 transcription factor normally control?
The yeast transcription factor GAL4 is widely used in Drosophila genetics to misexpress genes that are under control of the yeast upstream activator sequence (UAS).
How does split GAL4 work?
(B) The Split Gal4 system uses two enhancers with activity in overlapping cell groups (red and blue circles) to target reporter or effector expression (green) to the intersection of the two groups. The intersectional logic of expression is shown schematically in the Venn diagram (left).