What is SILAC media?
SILAC (Stable isotope labelling with amino acids in cell culture) media is prepared specifically for labeling experiments involving the use of stable amino acid isotopes. Ideal for NMR (Nuclear Magnetic Resonance) or Mass Spec experiments for large scale proteomics.
What is advanced DMEM f12?
Advanced DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Ham’s F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology.
What are the components of Dmem?
Dulbecco’s Modified Eagle’s Medium (DMEM) is modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. Contains 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate.
Why is SILAC used?
SILAC (1) is used to quantify protein expression differences in up to 2 or 3 samples from cells.
What can SILAC tell you?
Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. It is a popular method for quantitative proteomics.
What is SILAC technique?
SILAC (stable isotope labeling by amino acids in cell culture) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling.
What is SILAC experiment?
In SILAC experiments, proteins are metabolically labeled by culturing cells in media containing normal and heavy isotope amino acids. This makes proteins from the light and heavy cells distinguishable by mass spectrometry (MS) after the cell lysates are mixed and the proteins separated and/or enriched.
Can I use DMEM instead of DMEM F12?
You can use DMEM: F12 (DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12) which can support greater growth rates. DMEM and F12 are often mixed to combine the higher concentrations of components in DMEM with the wider range of Ham’s F12 ingredients.
Why is it called DMEM?
DMEM (Dulbecco’s Modified Eagle’s Medium) was originally suggested as Eagle’s medium with a ‘Fourfold concentration of amino acids and vitamins’ by Renato Dulbecco and G. Freeman published in 1959.
What is KnockOut DMEM?
KnockOut™ D-MEM is a basal medium optimized for growth of undifferentiated embryonic and induced pluripotent stem cells (1). The osmolarity is optimized to approximate that of mouse embryonic tissue. Contains no L-glutamine. For Research Use Only. Not for use in diagnostic procedures.
Why is DMEM media used?
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
Why DMEM is used in cell culture?
DMEM is a liquid serum-free media and a variant of BME, or Basal Medium Eagle, modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM. DMEM’s composition makes it an ideal supplement for most cell culture media applications.