What are SPRI beads used for?
SPRI technology uses paramagnetic beads to selectively bind nucleic acids by type and size, and are used for high-performance isolation, purification, and cleanup protocols. This technology supports applications such as next-generation sequencing (NGS), Sanger sequencing, qPCR, ddPCR and microarrays.
How do SPRI beads bind DNA?
Each bead is made of polystyrene surrounded by a layer of magnetite, which is coated with carboxyl molecules. It is these that reversibly bind DNA in the presence of the “crowding agent” polyethylene glycol (PEG) and salt (20% PEG, 2.5M NaCl is the magic mix).
How do AMPure beads work?
AMPure beads work like this: DNA has a negatively charged phosphate backbone and in a solution with a lot of salt and polyethylene glycol (PEG) the DNA gets crowded out of solution. It ends up on the beads, and because they are magnetic you can collect them using a magnetic held on one side of the tube.
Do SPRI beads bind RNA?
Solid Phase Reversible Immobilization (SPRI) is a technique applied in high-performance isolation, purification, and cleanup procedures. The process uses SPRI magnetic beads by selectively binding nucleic acids (involving RNA and DNA) found in all organisms.
Are SPRI and AMPure beads the same?
What is the difference between AMPure XP and SPRIselect beads of Beckman? Beckman has two types of SPRi beads- AMPure and SPRIselect, the later are much more expensive. According to their data sheets Ampure XP is used for PCR product purification while SPRIselect is used for size selection.
Do AMPure beads bind RNA?
The reagent will bind DNA and RNA, however, RNAClean XP is manufactured under RNase-free conditions and is QC tested to be RNase free whereas AMPure XP is not. Therefore, RNAClean XP is the recommended product for cleaning up RNA.
Are AMPure beads SPRI beads?
SPRI beads are Agencourt AMPure XP beads (Cat.
What are AMPure beads made of?
AMPure XP beads are tiny magnetic particles suspended in a solution of water, salt, polyethylene glycol and Tris buffer.
Are SPRi and AMPure beads the same?
What do AMPure XP beads bind to?
DNA fragments
Agencourt AMPure XP utilizes an optimized buffer to selectively bind DNA fragments 100 bp and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure. The result is a more purified PCR product.
Can you vortex AMPure beads?
AMPure purification protocol V. 1
Remove beads from the fridge, equilibrate to the room temperature, mix thoroughly but do not vortex. Add desired ratio of AMPure beads to the purified sample and mix well by pipetting.