What is DyLight 488 and why is it essential?
Thermo Scientific DyLight Fluor Phosphine Reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
What is the closest excitation wavelength for DyLight 488?
492 nm
DyLight 488 is a fluorescent compound with an excitation peak at 492 nm and an emission peak at 519 nm.
What color is Cy5?
Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiD and its variants.
What wavelength is Tritc?
544 nm
TRITC (Tetramethylrhodamine-isothiocyanate) is a fluorescent compound with an excitation peak at 544 nm and an emission peak at 570 nm.
What is Cy3 and Cy5?
Cyanine Cy3 and Cy5 are fluorescent dyes with many applications in nonlinear optics, laser physics, and particularly in biomedical imaging and single-molecule spectroscopy. Both dyes adopt an all-trans configuration in its ground state (Figure 1) and can be easily attached to nucleic acids and proteins.
What color is Cy3?
Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.
What color is TRITC?
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
What Colour is Cy5?
Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
Is 6 Fam the same as FAM?
Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein. The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading.
Is Cy3 red or green?
Cy3 dye dashboard
The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
What color is CY2?
Multi-Channel Viewer
The following color scheme is suggested: blue for the Cy2 image, green for the Cy3 image, and red for the Cy5 image.
Is 6-FAM the same as FAM?
Is FAM light sensitive?
FAM is quite a stable fluorophor. It won’t degrade significantly under normal laboratory lighting overnight. Store your primer in a dark place – like the refrigerator where it belongs. Apart from that, proceed as normal.
What is the wavelength of FAM?
495 nm
6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm.
What color is Cy3 dye?
What does VIC dye stand for?
victoria
In fluorescent dye name “VIC”, an abbreviation “VIC” stands for “victoria”.
Is FAM same as FITC?
FITC and FAM are both derivatives of fluorescein. FITC is fluorescein with an isothiocyanate reactive group added, while 6-FAM is 6-carboxyfluorescein. FITC and 6-FAM have identical spectral characteristics. In experiments, 6-FAM performs exactly like FITC.
What is FAM in fluorescence?
FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5′ or 3′ end of oligos.
Are HEX and Vic the same?
HEX has Ex at 535 nm and Em at 556 nm; VIC has Ex at 538 nm and Em at 554 nm. No big difference between them. However, some scientists claimed HEX could cause trouble due to its lactone structure. However, both VIC and HEX have similar lactone structure.
Why do people prefer Alexa Fluor 488 rather than FITC?
In addition, Alexa Flour 488 is pH insensitive (over a broad pH range) and tends to be brighter compared to FITC on most instruments. These properties, i.e. increased sensitivity and environmental stability, make Alexa Flour 488 suitable for intracellular staining.
Can I use PE and FITC?
In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. In those cases the relative contribution of each fluorophore to the signal in a given detector must be determined (Figure 11).
ARE FAM and FITC the same?
Is Alexa Fluor 488 same as FITC?
Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. However, Alexa Flour 488 display higher photostability and is less susceptible to self-quenching.
Is GFP the same as FITC?
Green Fluorescent Protein (GFP) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC against GFP.
Can you use PE and FITC together?
FITC overlap a lot with Pe, don’t use them together on same cells.