What is Nonidet P-40 Substitute?
Nonidet® P40 (substitute) is a non-ionic, non-denaturing detergent suitable for solubilizing and isolation of membrane proteins. Has been used to solubilize cerebral GABA receptors. Also useful in solubilization of proteins and lipids of the scallop gill ciliary membrane and of membranes from breast cancer tumors.
What does Nonidet P-40 do?
Nonidet p-40 [substitute]
A nonionic surfactant useful in isolating and purifying membrane proteins A non-ionic nondenaturing detergent used for solubilizing proteins Specifications Can be used as a substitute for octylphenoxypolyethoxyethanol CMC: 0.29 HLB Number 13.1 Reagent Grade Refractive Index:…
Is NP-40 same as igepal?
Nonidet P-40 and IGEPAL CA-630 are the same, those are merely different brand names. According to the manufacturer, Sigma-Aldrich, the two compounds are “chemically indistinguishable”.
What is the name of NP-40?
nonyl phenoxypolyethoxylethanol
NP-40 (also known as Tergitol-type NP-40 and nonyl phenoxypolyethoxylethanol) is a commercially available detergent with CAS Registry Number 9016-45-9. NP-40 is an ethoxylated nonylphenol for non-ionic surfactants and can act as emulsifier and demulsifier agent.
What is NP-40 lysis buffer?
Description. NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting. It can also be used as a wash buffer for immunoprecipitation reactions.
How do you dilute NP-40?
Directions: 1) Mix 20 ml of NP-40 with 80 ml of ddH2O by stirring. 2) Store at 4°C. Note: Nonidet P-40 is chemically identical to Igepal-CA-630.
What is igepal used for?
IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.
How do you store NP-40?
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 12 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Does NP-40 denature proteins?
A weaker detergent such as NP-40 can be used to solubilize proteins but not denature them.
How do I make a NP-40 lysis buffer?
1. Prepare 1% NP40 lysis buffer (LB). (To make 50 ml of 1% NP40 lysis buffer, add 5 ml of 10% NP40, 500 μl of 1 mM EDTA, 1.5 ml of 150 mM NaCl, and 1 ml of 20 mM Tris–Cl, pH 7.4; and bring the total volume to 50 ml using dH2O.) 2.
What is NP 40 lysis buffer?
How do you store igepal?
It is recommended that IGEPAL CA products be stored in sealed containers and kept in a cool dry place. Avoid contact with concentrated forms of oxidizing or reducing agents.
Is NP-40 a buffer?
Does NP-40 break nuclear membrane?
These buffers can be used for co-IPs. NP-40 and Triton X-100 will not lyse nuclear membranes. After lysis, pellet the nuclei by centrifugation and transfer the supernatant to a new tube. If you wish to isolate both the nuclear and soluble fractions, resuspend the nuclear pellet in RIPA buffer.
What is NP-40 in lysis buffer?
NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay (Luminex), ELISA, and Western blotting. It can also be used as a wash buffer for immunoprecipitation reactions. Solubility. It is soluble in water. Notes.