How many cell lines are maintained by ATCC for public health research?
ATCC credible cells lead to incredible research results The cell biology collection includes more than 4,000 continuous cell lines available by species, tissue/disease types, and signaling pathways.
How do I get a cell line?
The simplest way to create a new cell line is to modify an existing one, a common strategy when an established line already comes close to meeting the requirements. Cells optimized to grow particular viruses or maximize recombinant protein production often come from such modifications.
Can you buy cell lines?
You can also buy cell lines from well-known life science brands such as Sigma or Thermo Fisher. [5] These companies typically have smaller collections, but they are usually partnered with big repositories as well.
What is L cell line?
It is the parental line for the L Wnt-3A cell line (ATCC CRL-2647) and the L Wnt-5A cell line (ATCC CRL-2814). The cell line named L Cells is closely related or identical to L-M(TK-) cells (ATCC CCL-1.3).
How are cell lines named?
Many cell line names consist of less than 4 letters (e.g. C2, S2) Cell line names often look like gene/protein names (e.g. MCF2) Some cell line names look like person names (e.g. Ishikawa) Some cell line names are very generic (e.g. adapted, focus, label)
Where is ATCC?
Manassas, Virginia
Our newly expanded headquarters and bioproduction facility is located in Manassas, Virginia, near Washington DC, and our FDA-registered biorepository facility and research and development center is located in Gaithersburg, Maryland.
What are MEF cells?
Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line. After several transmission, MEFs will senesce and finally die off.
How do you read cell line names?
Are cell lines immortal?
There are various immortal cell lines. Some of them are normal cell lines (e.g. derived from stem cells). Other immortalised cell lines are the in vitro equivalent of cancerous cells.
How do you isolate a MEF cell?
Making MEFs
- Place freshly harvested embryos in 10cm cell culture dish.
- Cover embryos in PBSA and remove placental and other maternal tissues.
- Cut away top of head (eye and above) and eviscerate removing all innards.
- Save head for DNA isolation for genotyping (or yolk sac)
- Place embryo body in separate 10cm plate.