Is E. coli ATCC 25922?
ATCC 25922 is a recommended reference strain for antibiotic susceptibility testing. It has been found that passage in broth often results in a change in MIC levels. Therefore, it is best to keep it on agar and to make stocks for storage immediately. Repeated passage is discouraged.
Is S epidermidis resistant to novobiocin?
Novobiocin resistance is intrinsic to S. saprophyticus and some other staphylococci, but it is uncommon in other clinically relevant Staphylococcus species, such as S. epidermidis. Therefore coagulase-negative staphylococci that are resistant to novobiocin are presumptively identified as Staphylococcus saprophyticus.
What is ATCC in cell culture?
2The American Type Culture Collection. The American Type Culture Collection (ATCC) is a private, nonprofit organization dedicated to the acquisition, preservation, authentication, and distribution—the “APAD” activities—of diverse biological materials.
Which Staphylococcus is sensitive to novobiocin?
saprophyticus into Staphylococcus aureus (which is naturally susceptible to novobiocin) and assess the novobiocin susceptibility of the resulting recombinant strain. The gyrB gene and ribosome-binding site were amplified by PCR from S.
What is the origin of ATCC 25922?
The strain ATCC 25922 is a commonly used quality control strain, particularly in antibody sensitivity assays and was originally isolated from a human clinical sample collected in Seattle and WA (1946). It is of serotype O6 and biotype 1.
What is Escherichia coli ATCC 25922?
Escherichia coli, a seemingly ubiquitous Gram-negative bacterium, is best known for its ability to cause food-borne outbreaks. The strain ATCC 25922 is a commonly used quality control strain, particularly in antibody sensitivity assays and was originally isolated from a human clinical sample collected in Seattle and WA (1946).
Is ATCC 25922 a good quality control strain?
The strain ATCC 25922 is a commonly used quality control strain, particularly in antibody sensitivity assays and was originally isolated from a human clinical sample collected in Seattle and WA (1946). It is of serotype O6 and biotype 1. A prior assembly of the genome is publicly available, however, it is in 116 contigs (1).