What is high molecular weight cytokeratins?
Cytokeratin, High Molecular Weight (Concentrate) Reacts with the 68 kDa, 58 kDa, 56.5 kDa and 50 kDa proteins corresponding to cytokeratins 1, 5, 10 and 14. The antibody labels squamous, ductal and complex epithelia.
What are the types of cytokeratins?
There are two types of cytokeratins: the low weight or the acidic type I cytokeratins and the high weight or basic or neutral type II cytokeratins. The high-molecular-weight cytokeratins or basic or neutral cytokeratins comprise numerous subtypes, namely CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8, and CK9.
What is CK positive?
Cytokeratin 5/6 is a positive marker for malignant pleural mesothelioma, found in more than three-fourths of cases. It is also found in certain types of lung cancers and breast cancers. Pathologists use cytokeratin 5/6 to stain cancer tissue samples.
How many cytokeratins are there?
Abstract. Cytokeratins, belonging to the intermediate filament (IF) protein family, are particularly useful tools in oncology diagnostics. At present, more than 20 different cytokeratins have been identified, of which cytokeratins 8, 18, and 19 are the most abundant in simple epithelial cells.
What is low molecular weight cytokeratins?
Micrograph showing low molecular weight cytokeratin (LMWCK) staining of intermediate trophoblast (placental tissue) and endometrial glands….Types.
| Basic CK (Type B / Class II) | Acidic CK (Type A / Class I) | |
|---|---|---|
| LMWCK “simple keratins” | CK-7 CK-8 | CK-18 CK-19 CK-20 |
What is cytokeratins function?
A type of protein found on epithelial cells, which line the inside and outside surfaces of the body. Cytokeratins help form the tissues of the hair, nails, and the outer layer of the skin. They are also found on cells in the lining of organs, glands, and other parts of the body.
What does CAM5 2 positive mean?
The monoclonal antibody CAM5. 2 was typically used to identify secretory epithelial (glandular epithelium) cells and epithelial tumors, such as colorectal cancer and pancreatic cancer. Additionally, CAM5. 2 is generally accepted to be suitable for detection of metastatic breast cancer in (sentinel) lymph nodes.
What does negative CK20 mean?
For example, a CK7-negative/CK20-positive phenotype is often associated with carcinomas of colorectal origin, whereas a CK7-positive/CK20-negative phenotype is seen in a wide variety of carcinomas, including carcinomas of the lung, breast, thyroid, pancreas, and female genital tract.
Which high molecular weight cytokeratins are associated with poor survival in NSCLC?
Consequently, the expression of a group of high molecular weight cytokeratins by means of the clone 34 β E12 that reacts with 1, 5, 10, and 14 was associated with a poorer overall survival of NSCLC, without taking into account the histopathological type. Y. Chen, T. Cui, L. Yang et al.,
What is the molecular weight of cytokeratins?
Cytokeratins may also be classified as low molecular weight (commonly 8, 18, and 19) and high molecular weight (commonly 1, 5, 10, and 14) [ 8 V. Karantza, “Keratins in health and cancer: more than mere epithelial cell markers,” Oncogene, vol. 30, no. 2, pp. 127–138, 2011.
Do Pan-cytokeratins correlate with high molecular weight cytokeratin?
A tendency to correlate with pan-cytokeratins expression (, ; Spearman test) was also evidenced, while no correlation with the expression of high molecular weight cytokeratin was detected (, ; Spearman test).
Which low molecular weight cytokeratins are associated with lung cancer?
Nevertheless, the majority of these work included low molecular weight cytokeratins such as CK8 [ 32 Y. Fukunaga, S. Bandoh, J. Fujita et al., “Expression of cytokeratin 8 in lung cancer cell lines and measurement of serum cytokeratin 8 in lung cancer patients,” Lung Cancer, vol. 38, no. 1, pp. 31–38, 2002.