What is pBR322 class 12th?
pBR322 is a 4,362-bp ds DNA plasmid cloning vehicle aimed to authorize the easy and rapid formation of cloned recombinant DNA portions. It has two antibiotic resistance genes, an origin of replication, and a multiplicity of functional restriction sites for cloning or subcloning restriction segments.
What is pBR322 in biology?
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolivar Zapata, the postdoctoral researcher and Raymond L. Rodriguez.
How many cloning sites are in pBR322?
pBR322 contains restriction sites for more than 40 restriction enzymes including BamHI, HindIII, SalI, PvuI, PvuII, PstI, EcoRI, ClaI.
Which statement is true for pBR322?
Which of the statement is true for pBR322? Explanation: pBR 322 is the man-made vector. It contains both ampicillin resistant and tetracycline resistant genes. The cloning site is also present in both of the genes.
Is pBR322 a restriction enzyme?
Abstract. I have derived a complete restriction map of pBR322 from the total nucleotide sequence of the plasmid. Most of the restriction sites also have been demonstrated empirically. The exact sizes of all restriction fragments and the relative positions of the cuts are presented.
What are the features of pBR322 vector?
pBR322 Vector
- View sequence details.
- pBR322 DNA is a commonly used plasmid cloning vector in E.
- The molecule is a double-stranded circle 4,361* base pairs in length (2).
- pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
Which is absent in pBR322?
pBR322 vector has restriction sites such as Hindlll, EcoRI, BamHI, Sall, Pvill, Pstl, Clal, ori (origin of replication). From the above discussion, we can conclude that pBR322 has a restriction site for Sall. And option ‘D’ says that Sall is not present in the pBR322 vector. So, our answer will be option ‘D’.
How many restriction enzymes are there in pBR322?
What is the promoter of pBR322?
Promoter P1 is artificially created by the ligation of two different DNA fragments to create pBR322. Promoter P2 in the same region as P1, but it is on the opposite strand and initiates transcription in the direction of the tetracycline resistance gene.
What is the role of selectable markers in pBR322?
In the cloning vector pBR322, ampicillin and tetracycline resistance genes are the selectable markers. The role they play is that they help in the selection of transformed cells from non transformed cells. They also help distinguish recombinant cells from non-recombinant cells.
What is the nomenclature of pBR322?
pBR322 was the first artificial cloning vector constructed by Bolivar and Rodriguez in 1977. The nomenclature of the pBR322 plasmid is as stated below: P – Plasmid, BR – Stands for Bolivar and Rodriguez who constructed this plasmid, 322 – Number given to distinguish this plasmid from the other plasmids developed in the same laboratory.
Where can I find the NCERT solutions for all classes?
The NCERT Solutions for all classes and subjects can be viewed online as well as downloaded from BYJU’S. The detailed answers for the questions present in the NCERT textbook are solved by subject experts at BYJU’S and are provided on the website for free.
How NCERT solutions can benefit students of class 1-12?
These NCERT Solutions are designed to benefit both students and teachers by providing chapter-wise additional problems focusing primarily on testing conceptual knowledge with applications. Here, we have provided some of the important ways in which the solutions of NCERT can benefit students of Class 1 to 12 in general.
What is the difference between pBR322 and pUC19?
Both pBR322 andpUC19 are popular plasmids that are used as E.Coli cloning vectors. The prefix ‘p’ in both the vectors denotes ‘plasmid’. They have several restriction sites. Let’s look at the difference between these two vectors. It was the first artificial cloning vector created in the laboratory by Francisco Bolivar and Raymond L.Rodriguez.