What is SK-OV-3 cell line?
SK-OV-3 is a human ovarian cancer cell line with epithelial-like morphology. These cells are resistant to tumor necrosis factor and to other cytotoxic drugs such as diphtheria toxin, cisplatin, and adriamycin. The SK-OV-3 cell line forms colonies in soft agar, which serves as a surrogate assay for tumorigenicity.
What does SK-OV-3 stand for?
SK-OV-3 (also known as SKOV-3; SK. OV. 3; SKOV3; Skov3 and SKO3) is an ovarian cancer cell line derived from the ascites of a 64-year-old Caucasian female with an ovarian serous cystadenocarcinoma.
What is MDA MB 468 cell line?
MDA-MB-468 is a cell line with epithelial morphology that was isolated from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast. These cells have applications in breast cancer and immuno-oncology research.
What is A2780 cell line?
A2780 is an ovarian cancer cell line that was established from an Ovarian endometroid adenocarcinoma tumour in an untreated patient. It was deposited with ECACC by Dr T H Ward, from the Cell Culture Unit of the Patterson Laboratories, at the Christie Hospital in Manchester.
What is hepg2 cell line?
Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.
What is A549 cell line?
A549 cells are lung carcinoma epithelial cells that constitute a cell line. A549 is one of the commonly used cell lines for a wide range of research applications. In this article, we will provide you with all the information you will need to work with these cells.
What is the difference between MDA-MB-231 and MDA MB 468?
MDA-MB-157, MDA-MB-231, and BT-549 cells are characterized as triple-negative/basal-B mammary carcinoma, while the MDA-MB-468 cells are characterized as triple-negative/basal-A mammary carcinoma.
What is the difference between MCF 7 and MDA-MB-231?
The MCF-7 cell line has functional estrogen and EGF receptors, is dependent on estrogen and EGF for growth, and is uninvasive, while MDA-MB-231 cells are a model for more aggressive, hormone-independent breast cancer.
What are A9 cells?
A9 (APRT and HPRT negative derivative of Strain L) is a cell line isolated from wild-type Strain L cells that’s sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).
What media is used for HepG2?
Medium: The Hep G2 [HEPG2] (HB-8065) cells require the Eagle’s Minimum Essential Medium (EMEM) (ATCC 30-2003) with 10% Fetal Bovine Serum (FBS) (ATCC 30-2020) that has not been heat inactivated.
What is the difference between HepG2 and Huh7?
In this way, HepG2 cells carry wild-type p53, whereas Hep3B and Huh7 cells have null and point mutations at p53 codon 220 respectively. Hep G2 cells mostly used for research to find level of cytotoxicity and cellular metabolism and drug metabolism in the induced cell line.
Why do we use A549 cells?
The A549 cells have been used to model the alveolar Type II pulmonary epithelium. Studies have shown that this can be particularly useful in research for studying the metabolic processing of lung tissue and for identifying mechanisms of drug delivery to the tissue.
Is A549 cell line adherent?
A549 cells are cultured in complete media consisting of Dulbecco’s MEM modified with 10% FBS. The cells can be grown as adherent or in suspension in vitro. The A549 cell line grows easily and cell count doubling time is typically 24-40 hours.
What is the difference between MCF-7 and MDA-MB-231?
Do MDA-MB-231 cells express HER2?
MDA-MB-231 cells express low levels of HER2. B, Levels of soluble HER2 shedding by multiple cancer cell lines as determined by ELISA.
Is MCF 7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
What is microglial cell line?
Microglia Cell Lines. Microglia cell lines are available from mouse, rat, macaque and human origin (Table 1). Most of these lines stem from primary microglia cultures derived of the brain or the spinal cord, which were immortalized by viral transduction with oncogenes (e.g., v-myc, v-raf, v-mil, SV40 T antigen).
What type of cell line is HepG2?
Why HepG2 is used?
Hepatic cells are important in the drug development process since the liver is the main detoxifying organ in the human body and mainly responsible for drug metabolism and drug-drug interactions. Hence, HepG2 cells are widely used for drug development and toxicity testing.
What is HepG2 cell line?
Are HepG2 cells adherent?
HepG2 cells have adherent properties and grow as monolayers in small aggregates. HepG2 can be grown successfully at a large scale and stimulated with human growth hormone. They are also capable of secreting many plasma proteins, such as transferrin, fibrinogen, plasminogen and albumin.
What type of cells are A549?
How long does it take A549 to stick to plate?
A549 cells doubling time is approximately 22 hours.
Why are MCF-7 cells used?
4.8.
MCF-7 cells are useful for in vitro breast studies because they retained several ideal characteristics particular to mammary epithelium, such as the processing of estrogen, in the form of estradiol, via estrogen receptors (ER) in the cell cytoplasm [28]. It is the first hormone responding breast cancer cell line.
Does MCF-7 have HER2?
Two particularly important members of this large family are the epidermal growth factor receptor (EGFR), which is activated by the epidermal growth factor (EGF), and the human epidermal growth factor receptor-2 (HER2), both present in MCF-7 cells (7).