What is the pH indicator of phenol red?
Phenol red, also known as phenolsulfonphthalein, is a pH indicator dye that exhibits a gradual transition from yellow to red over a pH range of 6.2 to 8.2 (Figure 2). Above 8.2 the dye turns a bright fuchsia color.
Is phenol red flammable?
Fire hazard : Flammable liquid and vapour.
What is phenol red an indicator for?
Phenol red (PR) is the standard pH indicator in various cell and tissue culture media, as it provides a quick check for the health of the culture. PR has also been used in multiple protocols to detect cellular hydrogen peroxide as well as peroxidase activity from human peroxidase enzymes.
Is phenol red hazardous?
Irritating to eyes, respiratory system and skin. Accidental ingestion of the material may be damaging to the health of the individual. Limited evidence exists that the substance may cause irreversible but non-lethal mutagenic effects following a single exposure.
What is universal pH indicator?
Universal indicator is a mixture of dyes that changes colour gradually over a range of pH from 4-14. The colour is used to indicate pH directly. The main components of a Universal indicator, in the form of a solution, are thymol blue, methyl red, bromothymol blue and phenolphthalein.
Is phenol red an acid base indicator?
It has a role as a two-colour indicator, an acid-base indicator and a diagnostic agent. It is a 2,1-benzoxathiole, a member of phenols, a sultone and an arenesulfonate ester. Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories.
How do you store phenol red?
Storage: Store in General Storage Area [Green Storage] with other items with no specific storage hazards. Store in a cool, dry, well-ventilated, locked store room away from incompatible materials. Use ventilation to keep airborne concentrations below exposure limits.
What happens if you ingest Bromothymol blue?
Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May be harmful if swallowed. Inhalation: May cause respiratory tract irritation. May be harmful if inhaled.
How do you make phenol red indicator?
Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). After the solution is effected, add sufficient water to produce 100 ml.
Is phenol red a carcinogen?
Carcinogenicity: Not listed as a carcinogen (ACGIH, IARC, NTP): Phenol Red, sodium Salt Mutagenicity: No additional information. Reproductive Toxicity: No additional information.
Can phenol red go down the drain?
Do not discard any phenol, chloroform, or TRIzol™ down the sink drain. Collect pipette tips, eppendorf tubes, gloves, and other materials contaminated with phenol, chloroform, or TRIzol™ in a sealable container separate from other hazardous chemical waste.
What are two pH indicators?
Perhaps the best-known pH indicator is litmus. Thymol Blue, Phenol Red, and Methyl Orange are all common acid-base indicators. Red cabbage can also be used as an acid-base indicator.
What is the difference between pH and universal indicator?
What is the difference between pH paper and Universal indicator? A universal indicator is a liquid/solution substance that is dropped into a sample and changes the substance according to the pH. pH paper is an indicator that changes depending on the substance in which it is dipped (acidic or basic).
What color does phenol red turn in a base?
bright pink color
Phenol red is an example of a pH indicator, and it gives the yellow color in acidic solution and bright pink color in alkaline solution.
Does phenol red dissolve in water?
Phenol red is slightly soluble in water (3 mg/ml) and in alcohol (4 mg/ml). 2 Phenol red is readily soluble in aqueous alkali hydroxides or carbonates with formation of a red colored solution.
Can you drink phenol red?
Gastrointestinal. Nausea, vomiting, abdominal pain, and diarrhea are common symptoms after exposure to phenol by any route. Ingestion of phenol can also cause severe corrosive injury to the mouth, throat, esophagus, and stomach, with bleeding, perforation, scarring, or stricture formation as potential sequelae.
Is Bromothymol blue safe to touch?
Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Storage: Store in a cool, dry place.
Is Bromothymol Blue a carcinogen?
as a carcinogen or potential carcinogen by OSHA. Signs & Symptoms of Exposure Skin Irritation.
What kind of chemical is phenol red?
pH indicator
Phenol red is a water-soluble dye used as a pH indicator, changing from yellow to red over pH 6.6 to 8.0, and then turning a bright pink color above pH 8.1. As such, phenol red can be used as a pH indicator dye in various medical and cell biology tests.
Is phenol toxic to humans?
Severe exposure can cause liver and/or kidney damage, skin burns, tremor, convulsions, and twitching. Workers may be harmed from exposure to phenol. The level of harm depends upon the dose, duration, and work being done.
Is phenol red toxic for cells?
The results indicated that phenol red, commonly employed in toxicological study, was toxic after being adsorbed and delivered into cells by CBs in a serum-free cell culture medium.
Is phenol red soluble in water?
Is phenol a hazardous waste?
Disposal. Phenol and phenol-contaminated materials should be disposed of as hazardous waste.
What is the best pH indicator?
What is the range of pH indicator?
It covers a pH range from less than 3 (red) to greater than 11 (violet). Intermediate colors include orange/yellow (pH 3 to 6), green (pH 7 or neutral), and blue (pH 8 to 11).