What is inoculum process?
Inoculum preparation involves obtaining the organisms in an optimal state that is compatible with inoculation into cell culture, tissue culture, media, and fermentors. The prime objective is usually to achieve a high level of viable biomass in a suitable physiological state for use as an inoculum.
What does the term inoculum mean?
An inoculum can be defined as the population of microorganisms or cells that is introduced in the fermentation medium or any other suitable medium.
What is inoculum with example?
In immunology, an example of inoculum is the introduction of the pathogen or a part of the pathogen into the host’s body to initiate the infection process (defined as the intimate contact of the pathogen with its host). Thus, the inoculant may be an intact bacterial cell, protozoan, virus, or viroid.
What is inoculum expansion?
Inoculum quality is a key element for establishing an efficient bioconversion process. The main objective in inoculation expansion process is to generate large volume of viable cells in the shortest time.
What are the types of inoculum?
Different methods of inoculation include stab culture, slant culture, agar culture and broth culture.
What is the importance of inoculum?
The inoculum source had a significant impact on both growth and pH tolerance of the bacterial community in the EL soil. These differences between the EL soil inoculated with UL soil and FL soil were correlated to structural changes, as evidenced by both PLFA and DGGE analyses on the soil.
What is inoculum in microbiology?
In biology, inoculum refers to the source material used for inoculation. Inoculum may refer to: In medicine, material that is the source of the inoculation in a vaccine. In microbiology, propagules: cells, tissue, or viruses that are used to inoculate a new culture.
What is the importance of inoculum development?
The main aim of Inoculum is preparation that helps in achieving the high level of biomass in physiological use and inoculums. In the food microbiology it has been used for the development step by microorganism from culture for the food fermentation for the production of food.
What are the two method of inoculation?
Typically, inoculation is performed via mechanical wounding or grafting. Mechanical inoculation includes cutting, slashing, and rubbing, and is the only procedure for fulfilling Koch’s postulates.
What is the purpose of inoculation?
Inoculation may be defined as the process of adding effective bacteria to the host plant seed before planting. The purpose of inoculation is to make sure that there is enough of the correct type of bacteria present in the soil so that a successful legume-bacterial symbiosis is established.
What are the sources of inoculums?
Sources of Inoculum
The inoculum sometimes is present right in the plant debris or soil in the field where the crop is grown; other times it comes into the field with the seed, transplants, tubers, or other propagative organs or it may come from sources outside the field.
How can we develop inoculum?
The process is a stepwise and gradual increase in scaling-up the volume of inoculum to the desired level, which includes preparation of bacterial suspension (either vegetative cells or spores) in sterile tap water and then to the broth or in case of fungi, their hyphae are transferred to the broth.
How do you measure inoculum?
If you intent is to establish by inoculation 50 liters at 10^5/ml, think you’ll need 500 ml of 10^7/ml inoculum in those 50 liters (i.e. 500ml inoculum in 49.5 liters). Consider: concentration x volume = concentration x volume.
Why is inoculum development important?