What is Nextera XT?
Nextera XT DNA Library generates sequencing-ready libraries from small genomes in less than 90 minutes with only 15 minutes of hands-on time and can produce longer insert sizes for sequencing (300bp – 1.5kb target insert size).
What is nextera Flex?
Nextera™ DNA Flex Library Preparation Kit. A fast, integrated workflow for a wide range of applications from human whole-genome sequencing to amplicons, plasmids, and microbial species. For Research Use Only. Not for use in diagnostic procedures.
What are dual index primers?
Product Description. The CleanPlex® Dual-Indexed PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex Panels to construct targeted libraries for sequencing on an Illumina NGS platform.
How does nextera work?
How does the Nextera XT Assay Work? The Nextera XT DNA Sample Preparation Kit uses an engineered transposome to simultaneously fragment and tag (“tagment”) input DNA, adding unique adapter sequences in the process. A limited-cycle PCR reaction uses these adapter sequences to amplify the insert DNA.
How much DNA do I need for Illumina sequencing?
≥ 10 µl
Amplicon/Metagenomics Requirements
| Target | Input | Volume |
|---|---|---|
| 16S-V4 | Metagenomic DNA | ≥ 10 µl |
| 16S-V3V4 | Metagenomic DNA | ≥ 10 µl |
| Other | Primary PCR product | ≥ 10 µl |
What are R1 and R2 Fastq?
For a single-read run, one Read 1 (R1) FASTQ file is created for each sample per flow cell lane. For a paired-end run, one R1 and one Read 2 (R2) FASTQ file is created for each sample for each lane. FASTQ files are compressed and created with the extension *.
What is single index and dual index?
Libraries are then pooled and sequenced, and index reads are used during downstream analysis to identify and separate the sample libraries. The choice between single indexing (where one index read is performed) or dual indexing (where both an index 1 read and index 2 read are required) depends on various factors.
What is an index sequence?
Indexed sequencing is a method that allows multiple libraries to be pooled and sequenced together. Indexing libraries requires the addition of a unique identifier, or index sequence, to DNA samples during library preparation.
What is NextEra transposase?
Nextera, developed by Epicentre (an Illumina company), is an alternative approach to streamline the workflow, improve turnaround time and reduce DNA input and increase throughput.
Is NextEra a good stock to buy?
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How many reads from NextSeq?
Reads Passing Filter
| NextSeq 550 System High-Output Kit | NextSeq 550 System Mid-Output Kit | |
|---|---|---|
| Single Reads | Up to 400 Million | Up to 130 Million |
| Paired-End Reads | Up to 800 Million | Up to 260 Million |
How many indexes are in the Nextera XT V2?
Nextera XT V2 Index Kit set A, B, C, or D (96 indexes, 384 samples each – combine all four for 384 index combinations) Nextera XT Index Kit (24 indexes, 96 samples)
What is the Nextera XT Plus kit?
Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatible with all Illumina sequencers.
What is the input sample size for Nextera XT?
Nextera XT supports ultra-low DNA input of only 1 ng. It supports a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded cDNA. Multiplexing of up to 384 samples per Nextera XT library is available for projects requiring greater throughput.
What sequencing primer boxes do I need for Nextera XT libraries?
Sequencing Nextera XT libraries on the HiSeq 1000/2000, HiSeq 1500/2500 in High Output mode, Genome Analyzer, or HiScanSQ systems requires one of the following sequencing primer boxes. (These primers are included in the SBS reagents for other sequencers). TruSeq Dual Index Sequencing Primer Box, Single-Read (FC-121-1003)