What is Zelenka?
Brilliant green (also known as zelyonka or zelenka) is one of the triarylmethane dyes. It is closely related to malachite green.
What is brilliant green made of?
Brilliant green, or ethanaminium, is a derivative of triarylmethane dye that has been used as a dye to color silk and wool. Due to its inhibitory actions against Gram positive microorganisms, brilliant green can be found in antiseptic products such as solutions and swabs used to prevent infection.
What is brilliant green used for?
brilliant green, also called emerald green, or malachite green G, a triphenylmethane dye of the malachite-green series (see malachite green) used in dilute solution as a topical antiseptic. Brilliant green is effective against gram-positive microorganisms. It has also been used to dye silk and wool.
Is brilliant green safe?
Brilliant Green (633-03-4)
Harmful if swallowed. Symptoms/injuries after eye contact : Causes serious eye irritation. Symptoms/injuries after ingestion : Swallowing a small quantity of this material will result in serious health hazard.
Where does the name Zelenka come from?
Czech Slovak and Croatian: derivative of zelený (Czech and Slovak) zelen (Croatian) ‘green’ possibly a nickname for someone who habitually dressed in green or who was ‘green’ in the sense of being immature or inexperienced or for someone with a sickly ‘greenish’ complexion.
Where is the name Zelenka from?
Czech
The name Zelenka is girl’s name of Czech origin meaning “green”.
How do you make brilliant green solution?
Brilliant green (C27H34N2O4S) by BDH Chemicals was used as received. The standard solution of BG (1,000 mg/L) was prepared by mixing 1 g of BG per 1,000 mL of distilled water. Further dilution of the mixture was carried out to prepare 10–50 mg/L solutions.
What is Congo red dye?
Congo Red (diazo dye) is recognized as a carcinogen because it contains an aromatic amine in its structure. The presence of aromatic structures makes azo dyes resistant to natural degradation. Dyes remain in the environment for a long period and have negative effects on the fauna and flora8,9,10,11,12.
What is BGLB broth?
Brilliant Green Bile Lactose Broth is formulated for presumptive identification and confirmation of coliform bacteria. Ox-bile and Brilliant green inhibit the growth of gram-positive bacteria including lactose fermenting Clostridia.
How do you make 0.1 brilliant green solution?
Suspend 46.0 grams in 1000 ml distilled water and heat just to boiling.DO NOT AUTOCLAVE. Cool below 45°C and add 20 ml iodine solution (iodine – 6 grams and potassium iodide – 5 grams in 20 ml distilled water) and 10 ml of 0.1% brilliant green solution. Mix well and dispense in 10 ml quantities.
Is Congo red toxic?
Congo Red is an azo dye, it is toxic to many organisms and is a suspected carcinogen and mutagen. To give it its full name it is the disodium salt of 3,3′-(1E,1’E)-biphenyl-4,4′-diylbis(diazene-2,1-diyl)bis(4-aminonaphthalene-1-sulfonate). It is a benzidine-based anionic disazo dye.
Why is Congo red used?
Congo red was formerly used to dye cotton but has been superseded by dyes more resistant to light and to washing. It is still used in histology to stain tissues for microscopic examination, and to serve as an acid-base indicator, since it turns red in the presence of alkalies and blue when exposed to acids.
What does EC broth stand for?
Escherichia coli Broth
EC Broth (Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples. It was the recommended medium of the American Public Health Association (APHA) and the AOAC.
What is the full form of BGLB?
Acronym. Definition. BGLB. Brilliant Green Lactose Bile. Copyright 1988-2018 AcronymFinder.com, All rights reserved.
What is Congo Red used for?
Why is it called Congo Red?
It’s believed that the marketing people at AGFA (a Berlin-based company, remember) came up with the name Congo Red for their new sensational dye because, to Europeans in 1885, the Congo was seen as an exotic place, a land of mystery, and the name ‘Congo’ was currently on the tip of every tongue due to the world-famous …
Why is it called Congo red?
What is the purpose of EC medium?
EC Medium EC Medium, otherwise known as EC Broth, is used to detect the presence of coliform bacteria in drinking water, wastewater, and foods. Developed by Hajna and Perryl, it was designed for coliform bacteria, specifically E. coli.
What is EC in microbiology?
EC Broth (Escherichia coli Broth) is a selective enrichment broth designed for the isolation of coliforms, including E. coli, from water and food samples.
What is MacConkey broth used for?
MacConkey Broth is used for cultivating gram-negative, lactose-fermenting bacilli in water, foods and pharmaceutical raw materials as a presumptive test for coliform organisms.
What is Congo red made of?
Congo Red is the sodium salt of benzidinediazo-bis-1-naphthylamine-4-sulfonic acid; a diazo dye that is red in alkaline solution and blue in acid solution and used especially as an indicator and as a biological stain.
What is Congo red used for?
What is Congo red stain used for?
Staining with Congo Red (CR) is a qualitative method used for the identification of amyloids in vitro and in tissue sections.
What does EC broth contain?
The formulation includes a buffered lactose broth and bile salts No. 3. The presence of bile salts inhibits the growth of spore formers and enterococci, but allows the growth of E. coli and coliforms.
What color is MacConkey agar?
Result Interpretation on MacConkey Agar
The red color is due to production of acid from lactose, absorption of neutral red and a subsequent color change of the dye when the pH of medium falls below 6.8.