Can you freeze fitc dextran?

Can you freeze fitc dextran?

Sterilization of fairly dilute dextran solutions (1–10 mg/mL) may be performed by filtration using 0.2 μm pore-diameter sterile filters. For long-term storage, divide the aqueous solution into aliquots and freeze at ≤–20°C. AVOID REPEATED FREEZING AND THAWING.

Is dextran Fixable?

Dextrans that do not contain a primary amine will not be fixed.

What is TMR dextran?

Recommendations. Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

What is rhodamine dextran?

Description. Rhodamine dextran (RD) is widely used as both an anterograde and retrograde tracer in neurons and for numerous other applications. It is biologically rather inert having α-1,6-linked glucose residues resistant to cleavage by most endogenous cellular glycosidases.

How do I store my FITC dextran?

Stored properly at 2-8 °C and protected from light FITC-dextran powders should be stable for a minimum of two to three years.

How do you store dextran?

Dextran fractions are stable for more than 5 years when stored as a dry substance Dextran powder in well-sealed containers at room temperature. Dextran powder will slowly absorb moisture when exposed to air or when stored in non-air tight containers. Dextran solutions may be sterilized by heating in an autoclave.

How do you dissolve dextran in water?

Solubility. Dextran fractions are freely soluble in water. To hasten dissolution of dextran powders, the powder should be added portion wise to warm (hot) water with vigorous stirring to avoid lump formation.

Is FITC cell permeable?

The limiting membrane of FITC-labeled endosomes contained a vacuolar proton pump (pHmin = 6.23 +/- 0.033) and a water channel (osmotic water permeability coefficient, Pf = 0.052 +/- 0.005 cm/s) and was highly permeable to ethylene glycol and urea but relatively impermeable to glucose.

Does dextran dissolve in water?

With the exception of the highest MW dextran, D5501 (MW range = 5 million to 40 million), dextrans are very water soluble. Sigma tests the solubility of dextrans at concentrations generally exceeding 30 mg/ml in water. Dextrans are also freely soluble in DMSO, formamide, ethylene glycol, and glycerol.

Can dextran be autoclaved?

Neutral-aqueous dextran solutions can be sterilized by autoclaving at 110-115 °C for 30 to 45 minutes.

Is dextran biodegradable?

As a biodegradable polymer, dextran (a polysaccharide consisting predominantly of 1,6-glucosidic linkages) is very attractive because of its colloidal biocompatibility and because it is already widely used in commercial drug formulations.

Is Tritc cell permeable?

TRITC-dextrans are primarily used for studying permeability and transport in cells and tissues (5). An added benefit is that measurements of the fluorescence provide quantitative data on transport and permeability of healthy and diseased tissues.

What is FITC dextran?

Fluorescein isothiocyanate dextran (FITC-d) is a 3–5 kDa marker used to measure tight junction permeability.

How do you dissolve dextran?

How do you sterilize dextran?

Neutral-aqueous dextran solutions can be sterilized by autoclaving at 110-115 °C for 30 to 45 minutes. 11 Dextran can be hydrolyzed by strong acids at high temperatures. The terminal reducing end group of dextran can be oxidized in alkaline solutions.

Is dextran a bioplastic?

Dextran is a complex branched glucan (polysaccharide derived from the condensation of glucose), originally derived from wine. IUPAC defines dextrans as “Branched poly-α-d-glucosides of microbial origin having glycosidic bonds predominantly C-1 → C-6”.
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Dextran.

Is dextran a natural polymer?

NATURAL POLYMERS: CELLULOSE, CHITIN, CHITOSAN, GELATIN, STARCH, CARRAGEENAN, XYLAN AND DEXTRAN.

How long do fluorescent dyes last?

​The adduct formed in labeled cells is inherited by daughter cells after cell division. Analysis of heterogeneous cell populations over time. Fluorescence is preserved upon formaldehyde fixation. Dye is retained for up to 9 generations after staining.

What wavelength is TRITC?

544 nm

TRITC (Tetramethylrhodamine-isothiocyanate) is a fluorescent compound with an excitation peak at 544 nm and an emission peak at 570 nm.

What is the size for FITC?

4.1.
FITC (fluorescein isothiocyanate) conjugated with dextran has a molecular weight of 4000–40,000 Da and, for sodium fluorescein, the molecular weight is around 376 Da (Wilhelm et al., 2011). Both of these compounds show fluorescence and are hydrophilic in nature; the excitation wavelength is between 490 and 520 nm.

Is dextran soluble in water?

Is dextran a synthetic polymer?

Dextrans. Dextran is a synthetic colloid consisting of mixture of glucose polymers derived from the action of Leuconostoc mesenteroides on sucrose and is currently available in 10% dextran 40 (40 kDa) and 6% dextran 70 (70 kDa).

Why is dextran used?

Dextran is a medication used in managing and treating various clinical conditions, including during hemorrhage, shock, surgical procedures, radiological imaging, antithrombotic administration, and ophthalmic relief of xerophthalmia.

What affects fluorescence lifetime?

It is affected by external factors, such as temperature, polarity, and the presence of fluorescence quenchers. Fluorescence lifetime is sensitive to internal factors that are dependent on fluorophore structure.

What are DAPI FITC Tritc?

The DAPI-FITC-TRITC triple band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI, FITC, and TRITC with minimal crossover (spectral bleed-through) between adjacent bands, and it can also be employed with other combinations of fluorescent probes that have …