What is HuH-7 5 cells?

What is HuH-7 5 cells?

The human hepatoma-derived HuH-7 cell line and its derivatives (Huh7. 5 and Huh7. 5.1) have been widely used as a convenient experimental substitute for primary hepatocytes. In particular, these cell lines represent host cells suitable for propagating the hepatitis C virus (HCV) in vitro.

What is HuH-7 cells?

HuH-7 (hereafter Huh7) is a permanent cell line established from male hepatoma tissue, which was surgically removed from a 57-year-old Japanese male in 1982 (Nakabayashi et al., 1982). Huh7 and its derivatives have been used as a convenient experimental substitute for primary hepatocytes.

Why is HuH-7 cells used?

Huh-7 Origin Huh-7 is highly susceptible to the hepatitis C virus (HCV), and it is often used as a model to investigate hepatoma and HCV. This cell line can be used for an HCV replicon system, which allows for an in vitro production of infectious HCV particles and development of drugs that combat HCV.

Are HuH-7 cells hepatocytes?

Huh-7 is a well differentiated hepatocyte derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male in 1982, which is an immortal cell line of epithelial-like tumorigenic cells, established by Nakabayshi, H.

What does HuH-7 stand for?

Huh7 is a type of human liver cell line that may be grown in the laboratory for research purposes.

What is the difference between HepG2 and HuH-7?

In this way, HepG2 cells carry wild-type p53, whereas Hep3B and Huh7 cells have null and point mutations at p53 codon 220 respectively. Hep G2 cells mostly used for research to find level of cytotoxicity and cellular metabolism and drug metabolism in the induced cell line.

How do you thaw huh7 cells?

1. Pre-warm growth medium in 37 °C water bath.
2. Thaw the cells in a 37 °C water bath (not completely, there should be a small visible ice clump inside the tube)
3. Transfer the cells into a 15 ml centrifuge tube containing 9 ml pre-warmed growth medium.
4. 7.1.

What is Hep3B?

1-7 [Hep 3B, Hep-3B, Hep3B] is a cell line exhibiting epithelial morphology that was isolated from liver tissue derived from an 8-year-old, Black youth with liver cancer. This cell line contains an integrated hepatitis B virus genome. Homo sapiens, human. Morphology epithelial Tissue Liver Disease.

How do I unfreeze my cell line?

Guidelines for thawing cells

1. Thaw frozen cells rapidly (< 1 minute) in a 37°C water bath.
2. Dilute the thawed cells slowly before you incubate them, using pre-warmed growth medium.
3. Plate thawed cells at high density to optimize recovery.
4. Always use proper aseptic technique and work in a laminar flow hood.

What is Hep 3B?

How long can frozen cells stay at?

Cell lines can be kept at 70C for a few weeks, but they can’t be recovered after 6 months. In a liquid nitrogen freezer, frozen cell lines are stable for a long time.

What happens to cells when frozen?

Ice crystals that are formed during the freeze-thaw process can cause cell membranes to rupture. Rapid freezing results in ice crystal formation in the outer parts of cells, which causes the interior of the cells to expand, pushing against the plasma membrane until the cell bursts.

How do you test for confluency?

Eye estimation may be the possible way to estimate. If you want a mathematical data, then you have to use a hemocytometer and trypan blue to count the cell and then with surface area of the flask/ plate you can make a standard curve form which confluency may be calculated.

What does confluency mean in cell culture?

Cell confluence is defined as the percentage of the surface area of two-dimensional (2D) culture that is covered with cells. Commonly confluence assessment is used to determine the point when cells need to be passaged. Properly timing this moment is essential to maintaining cell phenotype and culture quality.

What type of cell culture is Hep 3B?

1-7 [Hep 3B, Hep-3B, Hep3B] is a cell line exhibiting epithelial morphology that was isolated from liver tissue derived from an 8-year-old, Black youth with liver cancer. This cell line contains an integrated hepatitis B virus genome.

What is the difference between HepG2 and Huh7?

How do you maintain a Huh7 cell?

Subculture Protocol for HuH-7 Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%. Do not allow cell concentration to exceed 90% confluency, otherwise cells will decrease proliferation rates. The doubling time of HuH-7 cells is 24 hours.

What are HuH7 cells?

These cells are adherent to the surface of flasks or plates and typically grow as 2D monolayers. Although containing many mutations and INDELS, it is worthy to note the Huh7 cells have a point mutation in the p53 gene. The Huh-7 cell line (origin: human liver) is used to create the Cell Line Derived Xenograft (CDX) HuH-7 mouse model.

What is the karyotype of a female?

Female karyotype: Females have two X chromosomes instead of X and Y of males. Both the X chromosomes are the same in size and are metacentric. The typical normal female karyotype is shown in the figure below, Normal female karyotype- 46,XX. There is an interesting mechanism associated with female chromosomes- the X-chromosome inactivation.

What is the origin of Huh7?

HuH-7 Origin. HuH-7 is a well differentiated hepatocyte derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male in 1982. The line was established by Nakabayshi, H. and Sato, J. The HuH-7 cell line is an immortal cell line of epithelial-like, tumorigenic cells.

What is the point mutation in the Huh7 cell line?

Although containing many mutations and INDELS, it is worthy to note the Huh7 cells have a point mutation in the p53 gene. The Huh-7 cell line (origin: human liver) is used to create the Cell Line Derived Xenograft (CDX) HuH-7 mouse model.